Solid phase capturable dideoxynucleotides for multiplex genotyping using mass spectrometry
نویسندگان
چکیده
منابع مشابه
Solid phase capturable dideoxynucleotides for multiplex genotyping using mass spectrometry.
We report an approach using solid phase capturable biotinylated dideoxynucleotides (biotin-ddNTPs) in single base extension for multiplex genotyping by mass spectrometry (MS). In this method, oligonucleotide primers that have different molecular weights and that are specific to the polymorphic sites in the DNA template are extended with biotin-ddNTPs by DNA polymerase to generate 3'-biotinylate...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2002
ISSN: 1362-4962
DOI: 10.1093/nar/gnf084